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Promega one-glo firefly luciferase reagent
One Glo Firefly Luciferase Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/one-glo+luciferase+firefly+reagent/one+glotm+luciferase+assay+system/10__3390_slash_ijms26104489-266-13-17
Average 90 stars, based on 1 article reviews
one-glo firefly luciferase reagent - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: The assay plates were incubated for 5 hours at 37° C., followed by addition of One-Glo luciferase firefly reagent (Promega, Madison, WI) at 1:1 volume/well and luminescence was measured in an EnVision Multilabel Plate Reader (PerkinElmer, Santa Clara, CA).

Article Title: Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids
Article Snippet: The assay plates were incubated for 5 hours at 37 °C, followed by addition of One-Glo luciferase firefly reagent (Promega, Madison, WI) at 1:1 volume/well and luminescence was measured in an EnVision Multilabel Plate Reader (PerkinElmer, Santa Clara, CA).

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: HCT116 cells were grown to 90-95% confluency in T175 flasks containing complete RPMI medium and transfected in bulk using Lipofectamine 2000 (Invitrogen, Carlsbad, Calif.) with 70 μg of ISG54-luciferase reporter plasmid (Elim Biopharmaceuticals Inc., Hayward, Calif.).

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: Transfection of the cells was allowed to take place for 6 hours, after which the cells were harvested by treatment with 0.25% trypsin EDTA (Corning Inc., Corning, N.Y.).

Luciferase:

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: The assay plates were incubated for 5 hours at 37° C., followed by addition of One-Glo luciferase firefly reagent (Promega, Madison, WI) at 1:1 volume/well and luminescence was measured in an EnVision Multilabel Plate Reader (PerkinElmer, Santa Clara, CA).

Article Title: Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids
Article Snippet: The assay plates were incubated for 5 hours at 37 °C, followed by addition of One-Glo luciferase firefly reagent (Promega, Madison, WI) at 1:1 volume/well and luminescence was measured in an EnVision Multilabel Plate Reader (PerkinElmer, Santa Clara, CA).

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: HCT116 cells were grown to 90-95% confluency in T175 flasks containing complete RPMI medium and transfected in bulk using Lipofectamine 2000 (Invitrogen, Carlsbad, Calif.) with 70 μg of ISG54-luciferase reporter plasmid (Elim Biopharmaceuticals Inc., Hayward, Calif.).

Article Title: Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device
Article Snippet: Transfection of the cells was allowed to take place for 6 hours, after which the cells were harvested by treatment with 0.25% trypsin EDTA (Corning Inc., Corning, N.Y.).



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